Quickly find that inspire student learning. Plant cells have a cell … (In Art / Shutterstock) Membrane-bound organelles. The question is based on a false premise. There are two primary types of cells: eukaryotic cells and prokaryotic cells. At 0.1–5.0 µm in diameter, prokaryotic cells are significantly smaller than eukaryotic cells, which have diameters ranging from 10–100 µm (Figure 2). Plasmids are actually common among fungi and higher plants. New words. Though bacteria have plasmids, plasmids do not contain the genes that are essential … Generally, plasmids are present in bacteria and archaea. Eukaryotic Cells and Prokaryotic Cells . E. coli (prokaryotic), NIH 3T3 and HeLa cells (eukaryotic) have been used to study electrotransfection mechanisms. Eukaryotic cells are called so because they have a true nucleus.The nucleus, which houses DNA, is contained within a membrane and separated from other cellular structures. Prokaryotic cells, however, have no true nucleus.DNA in a prokaryotic cell is not separated from the … Eukaryotic cells contain many membrane-enclosed, large, complex organelles in the cytoplasm whereas prokaryotic cells do not contain these membrane-bound organelles. Eukaryotic cells are covered by a cell membrane, which is also called a plasma or a cytoplasmic membrane. The small size of prokaryotes allows ions and organic molecules that enter them to quickly spread to other parts of the cell. Structure of a prokaryotic cell. Eukaryotic and Prokaryotic Cells Eukaryotic Cells. As you say plasmids are used routinely in cell biology research on mammalian cells to add in new genes to the cell which then allows their function to be studied. This sits in the cytoplasm of the bacterial cell.. Furthermore, eukaryotic genomic DNA also exists as chromosomes. Eukaryotic cells contain many membrane-enclosed, large, complex organelles in the cytoplasm whereas prokaryotic cells do not contain these membrane-bound organelles. (2013). Members of the five kingdoms of living things (with the exception of monerans) have eukaryotic cells. The human genome has 46 chromosomes. Bacterial DNA – a circular chromosome plus plasmids. Each of these organelles does a special job. Therefore, it contains only one circular DNA molecule and various smaller DNA circlets (plasmids). Plasmid transfection into mammalian cells is fairly straightforward and the resultant cells can either express the plasmid DNA transiently (similar to bacteria) or incorporate the genetic material directly into the genome to form a stable transfection. * The first plasmid detected in a eukaryote was discovered in 1967 in Saccharomyces cerevisiae (brewers yeast), the “2 μm DNA”. 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